What is a karyotype?
A chart of chromosome cutouts arranged from largest to smallest, used to identify abnormalities in chromosome number and structure.
What is the size comparison between the wheat genome and the human genome?
The wheat genome is roughly five times the size of the human genome.
1/155
p.4
Human Karyotype and Chromosome Identification

What is a karyotype?

A chart of chromosome cutouts arranged from largest to smallest, used to identify abnormalities in chromosome number and structure.

p.8
Genetic Composition of Modern Wheat

What is the size comparison between the wheat genome and the human genome?

The wheat genome is roughly five times the size of the human genome.

p.5
Cytogenetics and Chromosome Analysis

How is the short arm of a chromosome denoted?

The short arm is denoted by the letter p.

p.5
Cytogenetics and Chromosome Analysis

How is the long arm of a chromosome denoted?

The long arm is denoted by the letter q.

p.4
Cytological Techniques for Chromosome Study

What is the purpose of staining chromosomes during cytological analysis?

To recognize each of the duplicated chromosomes by its size, shape, and banding pattern.

p.9
Polytene Chromosomes and Their Analysis

What is the chromocenter in Drosophila polytene chromosomes?

A body where all the centromeres congeal, with chromosome arms emanating out of it.

p.2
Cytogenetics and Chromosome Analysis

What is the significance of cytogenetics in medicine?

It is used to determine whether disease conditions are associated with chromosome abnormalities.

p.2
Cytological Techniques for Chromosome Study

What dyes were traditionally used to stain chromosome spreads before the late 1960s and early 1970s?

Feulgen’s reagent and aceto-carmine.

p.3
Cytological Techniques for Chromosome Study

What is Giemsa stain and how is it used in chromosome staining?

Giemsa stain is a mixture of dyes used to stain chromosomes after they are treated with trypsin. It interacts with the remaining proteins on the chromosomes to produce a reproducible pattern of bands.

p.8
Polyploidy in Plants and Its Significance

What is the result of fertilization involving diploid gametes?

Tetraploid zygotes, which survive because each parental set of chromosomes is balanced.

p.1
Genetic Composition of Modern Wheat

What are the advantages of the triple-hybrid wheat over its ancestors?

Larger grains, easier to harvest, and ability to grow in a wider range of conditions.

p.8
Polyploidy in Plants and Its Significance

What are allopolyploids?

Polyploids created by hybridization between different species, where the contributing genomes are qualitatively different.

p.5
Cytogenetics and Chromosome Analysis

What is an ideogram?

An ideogram is the pattern of bands within a chromosome.

p.5
Polyploidy in Plants and Its Significance

What is polyploidy?

Polyploidy is the condition of having extra sets of chromosomes.

p.7
Polyploidy in Plants and Its Significance

What is the significance of chromosome doubling in hybrids for tetraploid formation?

It allows meiosis to proceed in good order, enabling the production of euploid gametes.

p.10
Aneuploidy and Its Effects on Organisms

Why do trisomic plants almost always inherit their extra chromosome from the female parent?

Because aneuploid pollen does not compete well with euploid pollen during pollen tube growth.

p.10
Polyploidy in Plants and Its Significance

Under what condition might polyploids produced by chromosome doubling in interspecific hybrids be fertile?

If their constituent genomes segregate independently.

p.6
Polyploidy in Plants and Its Significance

What is a univalent in the context of polyploidy?

A univalent is a solitary chromosome that is left without a partner during meiosis in a polyploid organism.

p.1
Importance of Wheat in Global Agriculture

How many varieties of wheat have been developed?

More than 17,000 varieties.

p.4
Human Karyotype and Chromosome Identification

How many chromosomes do diploid human cells contain?

46 chromosomes—44 autosomes and two sex chromosomes (XX in females and XY in males).

p.7
Polyploidy in Plants and Its Significance

What is a trivalent in the context of polyploidy?

A trivalent is a formation where all three homologous chromosomes synapse, each partially paired with the others.

p.4
Human Karyotype and Chromosome Identification

What is the size comparison between the X and Y chromosomes?

The X chromosome is intermediate in size, and the Y chromosome is about the same size as chromosome 22.

p.10
Polytene Chromosomes and Their Analysis

In which phase of the cell cycle are the polytene chromosomes of Drosophila trapped?

Interphase.

p.8
Polyploidy in Plants and Its Significance

Why are polyploids arising from hybridizations between different species more likely to be fertile?

Because chromosomes from different species are less likely to interfere with each other’s segregation during meiosis.

p.9
Polytene Chromosomes and Their Analysis

Who published detailed drawings of polytene chromosomes in the 1930s?

C. B. Bridges.

p.2
Cytological Techniques for Chromosome Study

How does immersion in a hypotonic solution aid in cytological analysis?

It causes cells to swell, diluting their contents and spreading out the chromosomes for easier analysis.

p.3
Cytological Techniques for Chromosome Study

Why is the bright-dark banding pattern produced by Quinacrine significant?

It is highly reproducible and specific for each chromosome, allowing cytogeneticists to identify particular chromosomes and determine if a chromosome is structurally abnormal.

p.8
Genetic Composition of Modern Wheat

How many chromosomes are present in the gametes and somatic cells of modern bread wheat?

21 chromosomes in the gametes and 42 in the somatic cells.

p.1
Genetic Composition of Modern Wheat

What is the chromosomal basis for the improvements in triple-hybrid wheat?

Triple-hybrid wheat contains the chromosomes of each of its progenitors, making it an amalgamation of the genomes of three different species.

p.8
Polyploidy in Plants and Its Significance

What are autopolyploids?

Polyploids created by chromosome duplication within a single species, where a single genome has been multiplied to create extra chromosome sets.

p.7
Polyploidy in Plants and Its Significance

What is apomixis?

A mechanism of asexual reproduction involving a modified meiosis that produces unreduced eggs, which then form seeds.

p.10
Polytene Chromosomes and Their Analysis

Why are polytene chromosomes ideal for cytogenetic analysis?

Because they undergo successive rounds of chromosome replication without cell division, producing large chromosomes.

p.7
Polyploidy in Plants and Its Significance

What happens during meiosis in a triploid organism?

Two of the three homologues synapse, leaving a univalent free to move to either pole during anaphase, or all three homologues synapse forming a trivalent.

p.10
Polyploidy in Plants and Its Significance

What are polyploids and why are many of them sterile?

Polyploids contain extra sets of chromosomes and are often sterile because their multiple sets of chromosomes segregate irregularly in meiosis.

p.6
Polyploidy in Plants and Its Significance

What is the proportion of grasses that are polyploids?

About two-thirds of all grasses are polyploids.

p.1
History of Wheat Cultivation

Where did the cultivation of wheat originate?

In the Middle East, around 10,000 years ago.

p.1
Importance of Wheat in Global Agriculture

What percentage of the food calories consumed by the human population is accounted for by wheat?

More than 20 percent.

p.9
Polytene Chromosomes and Their Analysis

What occurs during polyploidization without the separation of sister chromatids?

Duplicated chromosomes pile up next to each other, forming a bundle of strands aligned in parallel, resulting in polytene chromosomes.

p.9
Polytene Chromosomes and Their Analysis

How many rounds of replication do Drosophila polytene chromosomes undergo?

About nine rounds of replication, producing approximately 500 copies in each cell.

p.4
Cytological Techniques for Chromosome Study

What does the bright green fluorescent dye target in chromosome painting?

DNA in only a few pairs of chromosomes.

p.5
Cytological Techniques for Chromosome Study

How many bands can cytogeneticists identify in the entire human karyotype using high-resolution Giemsa staining?

About 850 bands.

p.2
Cytogenetics and Chromosome Analysis

How did the development of microscopes and staining procedures impact cytogenetics?

It led to significant advancements in the study of chromosome number and structure.

p.2
Cytological Techniques for Chromosome Study

Why are cells in the middle of mitosis often used for cytological analyses?

Because chromosomes are most easily seen during this stage.

p.2
Cytological Techniques for Chromosome Study

How has the development of cell-culturing techniques expanded cytological studies?

It has made it possible to study chromosomes in other types of cells, such as human white blood cells.

p.6
Polyploidy in Plants and Its Significance

What proportion of known plant genera contain polyploid species?

One-half of all known plant genera contain polyploid species.

p.6
Polyploidy in Plants and Its Significance

What are some examples of polyploid ornamental garden plants?

Roses, chrysanthemums, and tulips.

p.3
Cytological Techniques for Chromosome Study

What is chromosome painting and how does it work?

Chromosome painting involves treating chromosome spreads with fluorescently labeled DNA fragments (probes) that bind to complementary DNA sequences in the chromosomes, creating colorful images that reveal the location of specific DNA sequences.

p.1
Genetic Composition of Modern Wheat

What is the scientific name of modern cultivated wheat?

Triticum aestivum.

p.5
Cytogenetics and Chromosome Analysis

How were chromosomes classified before banding and painting techniques?

Chromosomes were arranged into groups according to size, with the largest classified as group A, the next largest as group B, and so forth.

p.7
Polyploidy in Plants and Its Significance

Why are most triploid species completely sterile?

Because zygotes formed by fertilization with aneuploid gametes are almost certain to die.

p.7
Polyploidy in Plants and Its Significance

How is sterility in triploid species circumvented in agriculture and horticulture?

By propagating the species asexually through methods like cuttings, grafts, and bulbs.

p.2
Cytogenetics and Chromosome Analysis

What is the main activity of the discipline called cytogenetics?

The analysis of stained chromosomes.

p.10
Aneuploidy and Its Effects on Organisms

What did Albert Blakeslee and John Belling discover about aneuploidy in Jimson weed?

They found that altered phenotypes were caused by dominant factors transmitted primarily through the female, and each mutant strain had an extra chromosome.

p.5
Aneuploidy and Its Effects on Organisms

What is aneuploidy?

Aneuploidy is a numerical change in part of the genome, usually just a single chromosome.

p.2
Cytogenetics and Chromosome Analysis

What was the incorrect number of human chromosomes thought to be before the swelling technique was used?

48 chromosomes.

p.6
Polyploidy in Plants and Its Significance

What is one general effect of polyploidy on cell size?

Polyploidy generally increases cell size.

p.6
Polyploidy in Plants and Its Significance

Why are polyploid plants significant for humans?

Polyploid plants tend to produce larger seeds and fruits, providing greater yields in agriculture.

p.3
Cytological Techniques for Chromosome Study

What is the limitation of traditional chromosome staining techniques?

They do not allow a researcher to distinguish one chromosome from another unless the chromosomes are very different in size or in the positions of their centromeres.

p.3
Cytological Techniques for Chromosome Study

What is the role of a probe in chromosome painting?

A probe is a fluorescently labeled DNA fragment that binds to complementary DNA sequences in the chromosomes, labeling them with the fluorescent dye and revealing specific DNA sequences.

p.1
History of Wheat Cultivation

From which regions did the progenitors of modern wheat originate?

Syria, Iran, Iraq, and Turkey.

p.4
Human Karyotype and Chromosome Identification

What is the smallest autosome in the human karyotype?

Chromosome 21.

p.10
Polytene Chromosomes and Their Analysis

What system did Bridges create to describe the features of polytene chromosomes?

An alphanumeric directory of sites along the length of each chromosome.

p.7
Polyploidy in Plants and Its Significance

How do fertile tetraploids arise?

By chromosome duplication in a hybrid produced by crossing two different but related diploid species.

p.5
Polyploidy in Plants and Its Significance

What is the term for organisms with complete, or normal, sets of chromosomes?

Euploid.

p.10
Aneuploidy and Its Effects on Organisms

What is a trisomy?

A condition where an organism has an extra chromosome, resulting in three copies of that chromosome.

p.6
Polyploidy in Plants and Its Significance

What is polyploidy?

The presence of extra chromosome sets.

p.6
Polyploidy in Plants and Its Significance

Name some polyploid crop plants.

Wheat, coffee, potatoes, bananas, strawberries, and cotton.

p.1
Importance of Wheat in Global Agriculture

What is the annual total wheat production worldwide?

700 million metric tons.

p.8
Genetic Composition of Modern Wheat

How has modern bread wheat, Triticum aestivum, evolved?

Through two hybridization events followed by chromosome doubling, resulting in a hexaploid with three different chromosome sets.

p.9
Polytene Chromosomes and Their Analysis

What are polytene chromosomes and where are they most spectacularly found?

Polytene chromosomes are bundles of aligned chromatin fibers, most spectacularly found in the salivary glands of Drosophila larvae.

p.9
Polytene Chromosomes and Their Analysis

What causes the variation in the density of chromatin in polytene chromosomes?

Differential coiling along the length of the bundle causes variation in the density of the chromatin.

p.9
Polytene Chromosomes and Their Analysis

What happens when dyes are applied to polytene chromosomes?

The denser chromatin stains more deeply, creating a pattern of dark and light bands.

p.8
Polyploidy in Plants and Its Significance

How can polyploid cells arise in plants?

Through mitosis without cytokinesis or altered meiosis producing unreduced gametes.

p.7
Polyploidy in Plants and Its Significance

Why are some tetraploids able to produce many viable progeny?

Because they contain two distinct sets of chromosomes, each duplicated, allowing regular meiosis.

p.2
Cytological Techniques for Chromosome Study

What types of cells are traditionally used for cytological analyses?

Rapidly growing material such as animal embryos and plant root tips.

p.2
Cytogenetics and Chromosome Analysis

What is the correct number of human chromosomes?

46 chromosomes.

p.6
Polyploidy in Plants and Its Significance

How does polyploidy affect the overall size of an organism?

Polyploid species tend to be larger and more robust than their diploid counterparts.

p.1
Importance of Wheat in Global Agriculture

How many people rely on wheat as their principal food crop today?

More than a billion people.

p.4
Human Karyotype and Chromosome Identification

What is the largest autosome in the human karyotype?

Chromosome 1.

p.5
Cytogenetics and Chromosome Analysis

What does the centromere divide each chromosome into?

The centromere divides each chromosome into long and short arms.

p.4
Cytological Techniques for Chromosome Study

What does the pink fluorescent dye target in chromosome painting?

The centromeres of each of the chromosomes.

p.9
Polytene Chromosomes and Their Analysis

What is the significance of the banding pattern in polytene chromosomes?

The banding pattern is highly reproducible, permitting detailed analysis of chromosome structure.

p.2
Cytogenetics and Chromosome Analysis

Who were the pioneers of cytogenetics?

Nineteenth-century European biologists who discovered chromosomes and observed their behavior during mitosis, meiosis, and fertilization.

p.9
Polytene Chromosomes and Their Analysis

What is the difference between euchromatin and heterochromatin in polytene chromosomes?

Euchromatin contains most of the genes and forms banded chromosome arms, while heterochromatin is gene-poor, surrounds the centromere, and does not become polytene.

p.5
Chromosome Rearrangements and Their Implications

What are chromosome rearrangements?

Chromosome rearrangements are structural changes in chromosomes, such as a piece of one chromosome being fused to another or a segment within a chromosome being inverted.

p.2
Cytological Techniques for Chromosome Study

What is the purpose of treating dividing cells with a chemical that disables the mitotic spindle?

To trap the chromosomes in mitosis, making them easier to see.

p.6
Polyploidy in Plants and Its Significance

Why is polyploidy rare in animals?

Polyploidy is rare in animals because it interferes with the sex-determination mechanism.

p.6
Polyploidy in Plants and Its Significance

What happens to zygotes formed from aneuploid gametes in polyploid species?

The resulting zygotes almost always die, leading to reduced fertility in polyploid species.

p.1
Importance of Wheat in Global Agriculture

How many varieties of wheat have been developed?
A) 1,000
B) 5,000
C) 10,000
D) 17,000
E) 25,000

D) 17,000
Explanation: More than 17,000 varieties of wheat have been developed, each adapted to different localities around the world.

p.3
Cytological Techniques for Chromosome Study

What is Quinacrine and how does it work in chromosome staining?

Quinacrine is a chemical relative of the antimalarial drug quinine. It stains chromosomes to show a characteristic pattern of bright bands on a darker background when exposed to ultraviolet (UV) light.

p.4
Cytological Techniques for Chromosome Study

What technique is used to uniquely identify each pair of human chromosomes?

Chromosome painting with probes made from human DNA fragments labeled with different fluorescent dyes.

p.5
Cytological Techniques for Chromosome Study

What techniques have made it possible to distinguish each human chromosome?

Banding and painting techniques.

p.7
Aneuploidy and Its Effects on Organisms

What is the result of chromosome separation during anaphase in triploid species?

It yields aneuploid cells.

p.8
Polyploidy in Plants and Its Significance

What is endomitosis?

A process that produces polyploid cells by chromosome duplication followed by separation of sister chromatids without cell division, resulting in extra chromosome sets within a single nucleus.

p.9
Polytene Chromosomes and Their Analysis

What happens to homologous polytene chromosomes in Drosophila?

They pair precisely, point-for-point along their length, making the banding patterns of each perfectly aligned.

p.10
Aneuploidy and Its Effects on Organisms

What is aneuploidy?

A numerical change in part of the genome, usually a change in the dosage of a single chromosome.

p.10
Aneuploidy and Its Effects on Organisms

What is the difference between hypoploid and hyperploid organisms?

Hypoploid organisms have an underrepresented chromosome or chromosome segment, while hyperploid organisms have an overrepresented chromosome or chromosome segment.

p.6
Polyploidy in Plants and Its Significance

How common is polyploidy in plants compared to animals?

Polyploidy is very common in plants but very rare in animals.

p.6
Polyploidy in Plants and Its Significance

Why are some polyploid species sterile?

Extra sets of chromosomes may segregate irregularly in meiosis, leading to unbalanced (aneuploid) gametes.

p.9
Polytene Chromosomes in Drosophila

What is the term used to describe chromosomes that form a bundle of strands aligned in parallel due to polyploidization without the separation of sister chromatids?
A) Aneuploid
B) Polytene
C) Diploid
D) Haploid
E) Monoploid

B) Polytene
Explanation: Polytene chromosomes are formed when duplicated chromosomes pile up next to each other, creating a bundle of strands aligned in parallel. The term 'polytene' comes from Greek words meaning 'many threads.'

p.5
Human Karyotype and Chromosome Identification

What was the primary challenge in distinguishing human chromosomes before the advent of banding and painting techniques?
A) Lack of microscopes
B) Chromosomes were too small
C) Chromosomes could only be grouped by size
D) Chromosomes were invisible
E) Chromosomes were too similar in shape

C) Chromosomes could only be grouped by size
Explanation: Before banding and painting techniques, cytogeneticists could only arrange chromosomes into groups according to size, making it nearly impossible to identify individual chromosomes within these groups.

p.10
Polytene Chromosomes in Drosophila

What is the primary use of Bridges' alphanumeric system in cytogenetics?
A) To identify species of insects
B) To describe the features of polytene chromosomes
C) To classify different types of plants
D) To measure the length of chromosomes
E) To analyze human chromosomes

B) To describe the features of polytene chromosomes
Explanation: Bridges' alphanumeric system is used to describe the features of polytene chromosomes, which are particularly detailed and thorough in cytogenetic analyses.

p.2
Cytological Techniques for Studying Chromosomes

Which dye was traditionally used to stain chromosomes before the late 1960s?
A) Hematoxylin
B) Feulgen’s reagent
C) Safranin
D) Crystal violet
E) Methylene blue

B) Feulgen’s reagent
Explanation: Before the late 1960s, chromosomes were usually stained with Feulgen’s reagent, a purple dye that reacts with the sugar molecules in DNA, or with aceto-carmine, a deep red dye.

p.1
Importance of Wheat in Global Agriculture

What percentage of the food calories consumed by the human population is accounted for by wheat?
A) 10%
B) 15%
C) 20%
D) 25%
E) 30%

C) 20%
Explanation: Wheat accounts for more than 20 percent of the food calories consumed by the entire human population, highlighting its importance as a staple food crop.

p.8
Polyploidy and Its Role in Plant Evolution

How does chromosome doubling contribute to the formation of polyploids?
A) By reducing the number of chromosomes
B) By preventing cell division
C) By duplicating chromosomes without cytokinesis
D) By eliminating chromosomes
E) By halving the chromosome number

C) By duplicating chromosomes without cytokinesis
Explanation: Chromosome doubling can occur when a cell undergoes mitosis without cytokinesis, leading to twice the usual number of chromosomes and potentially forming polyploid cells.

p.7
Polyploidy and Its Role in Plant Evolution

Why are most triploid species completely sterile?
A) They cannot undergo mitosis
B) They produce aneuploid gametes
C) They lack centromeres
D) They have too many chromosomes
E) They cannot photosynthesize

B) They produce aneuploid gametes
Explanation: Triploid species are usually sterile because the irregular separation of chromosomes during meiosis results in aneuploid gametes, which leads to non-viable zygotes.

p.5
Cytological Techniques for Studying Chromosomes

What is the role of high-resolution Giemsa staining in cytogenetics?
A) To create banding patterns for identifying individual chromosomes
B) To measure chromosome length
C) To detect genetic mutations
D) To separate chromosomes during cell division
E) To fuse chromosomes together

A) To create banding patterns for identifying individual chromosomes
Explanation: High-resolution Giemsa staining is used to create banding patterns that are useful in identifying individual chromosomes within a cell.

p.10
Polyploidy and Its Role in Plant Evolution

What is a key characteristic of polyploids?
A) They contain extra sets of chromosomes
B) They are always fertile
C) They are found only in humans
D) They have fewer chromosomes than diploids
E) They are always sterile

A) They contain extra sets of chromosomes
Explanation: Polyploids contain extra sets of chromosomes, which can lead to sterility due to irregular segregation during meiosis, although some may be fertile if their genomes segregate independently.

p.1
History of Wheat Cultivation

Where did the cultivation of wheat originate?
A) South America
B) Middle East
C) North America
D) East Asia
E) Australia

B) Middle East
Explanation: The cultivation of wheat originated some 10,000 years ago in the Middle East, which includes regions such as Syria, Iran, Iraq, and Turkey.

p.1
Chromosomal Structure and Hybridization in Wheat

What is the modern cultivated wheat known as?
A) Triticum durum
B) Triticum monococcum
C) Triticum aestivum
D) Triticum turgidum
E) Triticum spelta

C) Triticum aestivum
Explanation: Modern cultivated wheat is known as Triticum aestivum, which is a hybrid of at least three different species.

p.8
Polyploidy and Its Role in Plant Evolution

What is endomitosis?
A) A process where cells divide without duplicating chromosomes
B) A process where chromosomes duplicate without cell division
C) A process where cells undergo meiosis instead of mitosis
D) A process where cells lose chromosomes during division
E) A process where cells fuse together

B) A process where chromosomes duplicate without cell division
Explanation: Endomitosis involves chromosome duplication followed by separation of sister chromatids without accompanying cell division, leading to polyploid cells.

p.7
Polyploidy and Its Role in Plant Evolution

What is apomixis?
A) Sexual reproduction involving two parents
B) A type of asexual reproduction involving modified meiosis
C) A method of cloning animals
D) A process of genetic mutation
E) A technique for DNA sequencing

B) A type of asexual reproduction involving modified meiosis
Explanation: Apomixis is a form of asexual reproduction where modified meiosis produces unreduced eggs that form seeds, leading to new plants without fertilization.

p.10
Aneuploidy and Its Genetic Implications

What is aneuploidy?
A) A numerical change in the entire genome
B) A numerical change in part of the genome
C) The presence of polytene chromosomes
D) The absence of chromosomes
E) The doubling of chromosome sets

B) A numerical change in part of the genome
Explanation: Aneuploidy describes a numerical change in part of the genome, usually involving the dosage of a single chromosome or chromosome segment.

p.2
Cytological Techniques for Studying Chromosomes

What is the purpose of using a chemical to disable the mitotic spindle in cytological analysis?
A) To enhance cell growth
B) To trap chromosomes in mitosis
C) To stain the chromosomes
D) To fix the cells
E) To stimulate cell division

B) To trap chromosomes in mitosis
Explanation: The chemical that disables the mitotic spindle is used to trap the chromosomes in mitosis, making them most easily seen for cytological analysis.

p.6
Polyploidy and Its Role in Plant Evolution

What is polyploidy?
A) The presence of extra chromosome sets
B) The absence of chromosomes
C) The presence of a single chromosome set
D) The duplication of a single chromosome
E) The deletion of chromosome sets

A) The presence of extra chromosome sets
Explanation: Polyploidy refers to the condition where an organism has extra sets of chromosomes, which is common in plants but rare in animals.

p.6
Polyploidy and Its Role in Plant Evolution

What is a general effect of polyploidy on cell size?
A) Decreases cell size
B) No effect on cell size
C) Increases cell size
D) Reduces cell size by half
E) Makes cell size irregular

C) Increases cell size
Explanation: One general effect of polyploidy is that it increases cell size, which often correlates with an overall increase in the size of the organism.

p.9
Polytene Chromosomes in Drosophila

How many rounds of replication do Drosophila polytene chromosomes typically undergo?
A) 3
B) 5
C) 7
D) 9
E) 11

D) 9
Explanation: Drosophila polytene chromosomes typically undergo about nine rounds of replication, producing approximately 500 copies in each cell.

p.5
Cytological Techniques for Studying Chromosomes

What is an ideogram in cytogenetics?
A) A type of chromosome
B) A pattern of bands within a chromosome
C) A method for staining chromosomes
D) A type of genetic disorder
E) A chromosome rearrangement

B) A pattern of bands within a chromosome
Explanation: An ideogram is the pattern of bands within a chromosome, which can be identified using high-resolution staining techniques.

p.2
Cytogenetics and Chromosome Analysis Techniques

Which nineteenth-century discovery laid the foundation for cytogenetics?
A) Discovery of DNA structure
B) Observation of chromosomes during mitosis, meiosis, and fertilization
C) Identification of RNA
D) Discovery of cell organelles
E) Invention of the electron microscope

B) Observation of chromosomes during mitosis, meiosis, and fertilization
Explanation: The roots of cytogenetics trace back to the nineteenth-century European biologists who discovered chromosomes and observed their behavior during mitosis, meiosis, and fertilization.

p.3
Cytological Techniques for Studying Chromosomes

What is the characteristic feature of chromosomes stained with quinacrine?
A) They appear uniformly dark
B) They show a pattern of bright bands on a darker background
C) They become invisible under UV light
D) They show a pattern of dark bands on a bright background
E) They emit energy in the visible spectrum

B) They show a pattern of bright bands on a darker background
Explanation: Chromosomes stained with quinacrine show a characteristic pattern of bright bands on a darker background when exposed to ultraviolet (UV) light.

p.4
Human Karyotype and Chromosome Identification

How many chromosomes are present in diploid human cells?
A) 23
B) 44
C) 46
D) 22
E) 48

C) 46
Explanation: Diploid human cells contain a total of 46 chromosomes, which include 44 autosomes and 2 sex chromosomes (XX in females and XY in males).

p.6
Polyploidy and Its Role in Plant Evolution

Which of the following is NOT a polyploid crop plant?
A) Wheat
B) Coffee
C) Potatoes
D) Bananas
E) Corn

E) Corn
Explanation: While wheat, coffee, potatoes, and bananas are examples of polyploid crop plants, corn is not typically classified as polyploid.

p.9
Polytene Chromosomes in Drosophila

Where are the most spectacular examples of polytene chromosomes found?
A) Human liver cells
B) Plant root cells
C) Drosophila larvae salivary glands
D) Bacterial cells
E) Frog egg cells

C) Drosophila larvae salivary glands
Explanation: The most spectacular examples of polytene chromosomes are found in the salivary glands of Drosophila larvae, where each chromosome undergoes multiple rounds of replication.

p.5
Human Karyotype and Chromosome Identification

What does the letter 'p' denote in chromosome nomenclature?
A) The long arm of a chromosome
B) The centromere
C) The short arm of a chromosome
D) A specific gene
E) A chromosome group

C) The short arm of a chromosome
Explanation: The letter 'p' denotes the short arm of a chromosome, derived from the French word 'petite,' meaning 'small.'

p.2
Cytogenetics and Chromosome Analysis Techniques

What is the main activity of the discipline called cytogenetics?
A) Studying protein synthesis
B) Analyzing stained chromosomes
C) Observing cell membranes
D) Measuring DNA replication speed
E) Examining RNA sequences

B) Analyzing stained chromosomes
Explanation: Cytogenetics primarily involves the analysis of stained chromosomes to study their number and structure, which is crucial for understanding genetic information and abnormalities.

p.3
Cytological Techniques for Studying Chromosomes

What is the primary limitation of using uniform stains for chromosome analysis?
A) They are too expensive
B) They do not allow researchers to distinguish one chromosome from another
C) They are harmful to the chromosomes
D) They require special equipment
E) They are not reproducible

B) They do not allow researchers to distinguish one chromosome from another
Explanation: Uniform stains do not allow researchers to distinguish one chromosome from another unless the chromosomes are very different in size or in the positions of their centromeres.

p.4
Cytological Techniques for Studying Chromosomes

What is the purpose of using two different human DNA fragments labeled with different fluorescent dyes in chromosome painting?
A) To measure the length of chromosomes
B) To identify the number of chromosomes
C) To distinguish between different chromosomes
D) To determine the age of chromosomes
E) To analyze the genetic sequence of chromosomes

C) To distinguish between different chromosomes
Explanation: The use of two different human DNA fragments labeled with different fluorescent dyes allows for the identification of specific chromosomes by making them stand out with distinct colors, thus aiding in distinguishing between different chromosomes.

p.4
Cytological Techniques for Studying Chromosomes

What is the significance of the banding pattern on chromosomes?
A) It indicates the age of the chromosome
B) It helps in identifying each chromosome
C) It shows the length of the chromosome
D) It reveals the genetic sequence
E) It determines the function of the chromosome

B) It helps in identifying each chromosome
Explanation: The banding pattern on chromosomes, when stained appropriately, allows for the recognition and identification of each chromosome based on its size, shape, and unique banding pattern.

p.8
Polyploidy and Its Role in Plant Evolution

What is the difference between allopolyploids and autopolyploids?
A) Allopolyploids have chromosomes from the same species, autopolyploids have chromosomes from different species
B) Allopolyploids have chromosomes from different species, autopolyploids have chromosomes from the same species
C) Allopolyploids have fewer chromosomes than autopolyploids
D) Allopolyploids are always sterile, autopolyploids are always fertile
E) Allopolyploids are found only in animals, autopolyploids are found only in plants

B) Allopolyploids have chromosomes from different species, autopolyploids have chromosomes from the same species
Explanation: Allopolyploids are formed by hybridization between different species, while autopolyploids are formed by chromosome duplication within a single species.

p.7
Polyploidy and Its Role in Plant Evolution

How is sterility in triploid plants circumvented in agriculture?
A) By genetic modification
B) By asexual propagation
C) By cross-breeding with diploids
D) By increasing sunlight exposure
E) By reducing water intake

B) By asexual propagation
Explanation: Sterility in triploid plants is circumvented by asexual propagation methods such as cultivation from cuttings, grafts, and bulbs.

p.10
Polytene Chromosomes in Drosophila

Why are polytene chromosomes particularly useful for cytological analysis?
A) They are found in humans
B) They are trapped in the interphase of the cell cycle
C) They are only present during mitosis
D) They are found in all living organisms
E) They are smaller than other chromosomes

B) They are trapped in the interphase of the cell cycle
Explanation: Polytene chromosomes are trapped in the interphase of the cell cycle, allowing for detailed and thorough cytological analyses.

p.2
Human Karyotype and Chromosome Identification

What was the incorrect number of human chromosomes thought to be before the swelling technique was used?
A) 44
B) 46
C) 48
D) 50
E) 52

C) 48
Explanation: It was erroneously thought for many years that human cells contained 48 chromosomes. The correct number, 46, was determined only after the swelling technique was used to separate the chromosomes within individual mitotic cells.

p.4
Human Karyotype and Chromosome Identification

Which chromosome is the largest autosome in humans?
A) Chromosome 21
B) Chromosome 22
C) Chromosome 1
D) Chromosome X
E) Chromosome Y

C) Chromosome 1
Explanation: Chromosome 1 is the largest autosome in humans, while chromosome 21 is the smallest, and chromosome 22 is the second smallest.

p.1
Chromosomal Structure and Hybridization in Wheat

What is the chromosomal basis for the improvements in triple-hybrid wheat?
A) They contain chromosomes from only one species
B) They contain chromosomes from two species
C) They contain chromosomes from three different species
D) They have fewer chromosomes than their ancestors
E) They have no chromosomal differences

C) They contain chromosomes from three different species
Explanation: Triple-hybrid wheat contains the chromosomes of each of its progenitors, making it an amalgamation of the genomes of three different species, which contributes to its superior characteristics.

p.9
Polytene Chromosomes in Drosophila

What is the chromocenter in Drosophila polytene chromosomes?
A) A region of high gene activity
B) A body formed by the congealing of all centromeres
C) A site of DNA replication
D) A protein complex involved in chromosome segregation
E) A region of euchromatin

B) A body formed by the congealing of all centromeres
Explanation: In Drosophila polytene chromosomes, the chromocenter is a body formed by the congealing of all centromeres, with chromosome arms emanating from it.

p.5
Aneuploidy and Its Genetic Implications

What is the main difference between aneuploidy and polyploidy?
A) Aneuploidy affects the entire genome, polyploidy affects only one chromosome
B) Aneuploidy refers to a numerical change in part of the genome, polyploidy refers to a numerical change in a whole set of chromosomes
C) Aneuploidy is beneficial, polyploidy is harmful
D) Aneuploidy occurs in plants, polyploidy occurs in animals
E) Aneuploidy is a structural change, polyploidy is a numerical change

B) Aneuploidy refers to a numerical change in part of the genome, polyploidy refers to a numerical change in a whole set of chromosomes
Explanation: Aneuploidy implies a genetic imbalance due to a numerical change in part of the genome, usually just a single chromosome, whereas polyploidy refers to a numerical change in a whole set of chromosomes.

p.10
Aneuploidy and Its Genetic Implications

What is a hypoploid organism?
A) An organism with extra sets of chromosomes
B) An organism with a chromosome or chromosome segment underrepresented
C) An organism with a chromosome or chromosome segment overrepresented
D) An organism with polytene chromosomes
E) An organism with no chromosomes

B) An organism with a chromosome or chromosome segment underrepresented
Explanation: A hypoploid organism has a chromosome or chromosome segment that is underrepresented, leading to various abnormalities.

p.3
Cytological Techniques for Studying Chromosomes

What is the most advanced technique used by cytogeneticists today for chromosome analysis?
A) Quinacrine staining
B) Giemsa staining
C) Chromosome painting
D) Trypsin treatment
E) Uniform staining

C) Chromosome painting
Explanation: Chromosome painting is the most advanced technique used by cytogeneticists today, involving the use of fluorescently labeled DNA fragments to create colorful chromosome images.

p.4
Human Karyotype and Chromosome Identification

What is a karyotype?
A) A type of genetic mutation
B) A chart of chromosome cutouts arranged by size
C) A method for sequencing DNA
D) A type of cell division
E) A protein found in the nucleus

B) A chart of chromosome cutouts arranged by size
Explanation: A karyotype is a chart that displays chromosome cutouts arranged from largest to smallest, allowing for the identification of abnormalities in chromosome number and structure.

p.1
Chromosomal Structure and Hybridization in Wheat

What characteristic made the triple-hybrid wheats superior to their ancestors?
A) Smaller grains
B) More difficult to harvest
C) Grew in a narrower range of conditions
D) Larger grains and more easily harvested
E) Less adaptable to different environments

D) Larger grains and more easily harvested
Explanation: The triple-hybrid wheats were superior to their ancestors because they had larger grains, were more easily harvested, and grew in a wider range of conditions.

p.8
Polyploidy and Its Role in Plant Evolution

Why are polyploids arising from hybridizations between different species more likely to be fertile?
A) Because they have fewer chromosomes
B) Because chromosomes from different species do not interfere with each other’s segregation during meiosis
C) Because they have more chromosomes
D) Because they undergo mitosis more frequently
E) Because they are always diploid

B) Because chromosomes from different species do not interfere with each other’s segregation during meiosis
Explanation: Polyploids from hybridizations between different species (allopolyploids) are more likely to be fertile because the chromosomes from different species segregate more effectively during meiosis.

p.7
Polyploidy and Its Role in Plant Evolution

How do fertile tetraploids arise?
A) By spontaneous mutation
B) By chromosome duplication in a hybrid
C) By exposure to radiation
D) By hybridization with triploids
E) By genetic engineering

B) By chromosome duplication in a hybrid
Explanation: Fertile tetraploids arise when chromosomes in a hybrid, produced by crossing two different but related diploid species, are duplicated, allowing regular meiosis and the production of viable gametes.

p.10
Aneuploidy and Its Genetic Implications

What did Belling discover about the transmission of trisomic phenotypes in Datura?
A) They are transmitted equally through male and female
B) They are primarily transmitted through the male
C) They are primarily transmitted through the female
D) They are not transmitted at all
E) They are transmitted through pollen only

C) They are primarily transmitted through the female
Explanation: Belling discovered that trisomic phenotypes in Datura are primarily transmitted through the female due to the poor competition of aneuploid pollen with euploid pollen during pollen tube growth.

p.2
Cytological Techniques for Studying Chromosomes

Which type of cells are commonly used for cytological analysis due to their rapid growth?
A) Muscle cells
B) Neurons
C) Animal embryos and plant root tips
D) Skin cells
E) Liver cells

C) Animal embryos and plant root tips
Explanation: Rapidly growing materials such as animal embryos and plant root tips are traditionally used for cytological analysis because they provide a high number of dividing cells.

p.6
Polyploidy and Its Role in Plant Evolution

Why is polyploidy rare in animals?
A) It enhances sexual reproduction
B) It interferes with the sex-determination mechanism
C) It increases cell size
D) It leads to asexual reproduction
E) It is beneficial for animal growth

B) It interferes with the sex-determination mechanism
Explanation: Polyploidy is rare in animals because it likely interferes with the sex-determination mechanism, which is crucial for sexual reproduction.

p.8
Chromosomal Structure and Hybridization in Wheat

What is the chromosomal composition of modern bread wheat (Triticum aestivum)?
A) Diploid with 14 chromosomes
B) Tetraploid with 28 chromosomes
C) Hexaploid with 42 chromosomes
D) Octaploid with 56 chromosomes
E) Triploid with 21 chromosomes

C) Hexaploid with 42 chromosomes
Explanation: Modern bread wheat is a hexaploid containing three different chromosome sets, each duplicated, resulting in a total of 42 chromosomes in somatic cells.

p.7
Polyploidy and Its Role in Plant Evolution

What is a trivalent in the context of polyploidy?
A) A chromosome with three arms
B) A chromosome with three centromeres
C) A synapse involving three homologous chromosomes
D) A chromosome with three telomeres
E) A chromosome with three genes

C) A synapse involving three homologous chromosomes
Explanation: In polyploidy, a trivalent refers to the synapse formation where three homologous chromosomes partially pair with each other during meiosis.

p.5
Human Karyotype and Chromosome Identification

What is the significance of the centromere in chromosome structure?
A) It determines the chromosome's length
B) It divides the chromosome into long and short arms
C) It is the site of genetic mutations
D) It is where chromosomes are stained
E) It is the end of the chromosome

B) It divides the chromosome into long and short arms
Explanation: The centromere divides each chromosome into long and short arms, which are denoted by the letters 'q' and 'p,' respectively.

p.2
Cytological Techniques for Studying Chromosomes

What is the purpose of using a hypotonic solution in cytological analysis?
A) To fix the cells
B) To stimulate cell division
C) To swell the cells by osmosis
D) To stain the chromosomes
E) To disable the mitotic spindle

C) To swell the cells by osmosis
Explanation: A hypotonic solution is used to swell the cells by osmosis, which helps in spreading out the chromosomes for easier analysis when the cells are squashed on a microscope slide.

p.3
Cytological Techniques for Studying Chromosomes

What is a DNA fragment used in chromosome painting often called?
A) Stain
B) Enzyme
C) Probe
D) Dye
E) Marker

C) Probe
Explanation: In chromosome painting, the DNA fragment used to bind to complementary DNA in the chromosomes is often called a probe.

p.6
Polyploidy and Its Role in Plant Evolution

Why are some polyploid species sterile?
A) They cannot undergo mitosis
B) They produce balanced gametes
C) They segregate chromosomes irregularly in meiosis
D) They have reduced cell size
E) They have increased fertility

C) They segregate chromosomes irregularly in meiosis
Explanation: Some polyploid species are sterile because extra sets of chromosomes may segregate irregularly in meiosis, leading to unbalanced (aneuploid) gametes, which often result in non-viable zygotes.

p.9
Polytene Chromosomes in Drosophila

What causes the variation in the density of chromatin along the length of polytene chromosomes?
A) Differential coiling
B) DNA methylation
C) Histone modification
D) RNA interference
E) Protein folding

A) Differential coiling
Explanation: Differential coiling along the length of polytene chromosomes causes variation in the density of the chromatin, leading to the creation of dark and light bands when stained.

p.5
Polyploidy and Its Role in Plant Evolution

What term describes organisms with extra sets of chromosomes?
A) Euploid
B) Aneuploid
C) Polyploid
D) Haploid
E) Diploid

C) Polyploid
Explanation: Organisms that carry extra sets of chromosomes are referred to as polyploid, which is described by the level of polyploidy using a basic chromosome number, usually denoted as 'n.'

p.2
Cytogenetics and Chromosome Analysis Techniques

What significant advancement in the twentieth century boosted cytogenetics research?
A) Discovery of antibiotics
B) Improvement of microscopes and staining procedures
C) Development of CRISPR technology
D) Invention of PCR
E) Discovery of the double helix structure of DNA

B) Improvement of microscopes and staining procedures
Explanation: The advancement of microscopes and better procedures for preparing and staining chromosomes significantly boosted cytogenetics research in the twentieth century.

p.3
Cytological Techniques for Studying Chromosomes

What is the purpose of treating chromosomes with trypsin before staining with Giemsa?
A) To make the chromosomes fluorescent
B) To remove some of the proteins associated with the chromosomes
C) To increase the size of the chromosomes
D) To make the chromosomes more uniform
E) To change the color of the chromosomes

B) To remove some of the proteins associated with the chromosomes
Explanation: Before staining with Giemsa, chromosomes are treated with trypsin, an enzyme that removes some of the proteins associated with the chromosomes, allowing the stain to interact with the remaining proteins.

Study Smarter, Not Harder
Study Smarter, Not Harder