What is the Central Dogma of Molecular Biology?
The process by which genetic information flows from DNA to RNA to protein.
How does the anticodon of tRNA interact with mRNA?
The anticodon matches with a codon on the mRNA.
1/37
p.1
Central Dogma of Molecular Biology

What is the Central Dogma of Molecular Biology?

The process by which genetic information flows from DNA to RNA to protein.

p.4
Translation Mechanism

How does the anticodon of tRNA interact with mRNA?

The anticodon matches with a codon on the mRNA.

p.1
Epigenetics and Gene Expression

What is epigenetics?

The study of heritable changes that alter gene expression without changing the primary DNA sequence.

p.3
Diseases Related to Mutated Splicing

What protein is reduced in individuals with Spinal Muscular Atrophy?

Survival of motor neuron (SMN) protein.

p.4
Translation Mechanism

What role does transfer RNA (tRNA) play in translation?

tRNA carries an amino acid corresponding to its anticodon.

p.3
Diseases Related to Mutated Splicing

What is Spinal Muscular Atrophy (SMA)?

An autosomal recessive disease characterized by progressive muscle weakening due to the loss of motor neurons.

p.1
MicroRNA Functionality

What is microRNA (iRNA)?

Single-stranded RNA with a length of 18-25 nucleotides that regulates gene expression at a post-translational level.

p.3
Diseases Related to Mutated Splicing

What role does the mutation in SMN2 play in splicing?

It blocks a helper protein involved in splicing.

p.1
MicroRNA Functionality

How does microRNA regulate gene expression?

By binding to mRNA to either destroy and recycle its components or preserve them for later translation.

p.1
Reverse Transcription in Viruses

What is reverse transcription?

The process of converting RNA back to DNA, primarily seen in viruses like HIV.

p.4
Translation Mechanism

What happens after mRNA reaches the ribosomes?

The small and large subunits assemble around the mRNA.

p.3
Diseases Related to Mutated Splicing

What is the key mutation in the SMN2 gene?

A C to T mutation.

p.3
Diseases Related to Mutated Splicing

What is the consequence of the shorter SMN protein produced in SMA?

It cannot function properly and is broken down.

p.3
Diseases Related to Mutated Splicing

Which genes code for the SMN protein?

SMN1 and SMN2.

p.3
Diseases Related to Mutated Splicing

What happens to the mature mRNA in affected individuals with SMA?

It lacks exon 7 due to a mutation in SMN2, leading to a shorter, non-functional SMN protein.

p.3
Diseases Related to Mutated Splicing

How does the functionality of SMN1 compare to SMN2?

SMN1 is fully functional, while SMN2 is only partially functional.

p.4
Translation Mechanism

What is the first step in the translation process?

mRNA leaves the nucleus and travels to the ribosomes in the cytoplasm.

p.1
Central Dogma of Molecular Biology

Who proposed the Central Dogma?

Francis Crick.

p.4
Translation Mechanism

What are the binding sites for tRNA on the ribosome?

A-site, P-site, and E-site.

p.3
Diseases Related to Mutated Splicing

On which chromosome are the SMN1 and SMN2 genes located?

Chromosome 5.

p.4
Translation Mechanism

What occurs after the polypeptide chain is completed?

The completed polypeptide chain detaches from the ribosome and folds into conformation.

p.1
Translation Mechanism

What is the role of RNA in the translation process?

RNA is translated into protein.

p.1
Epigenetics and Gene Expression

What are epigenetic marks?

Chemical modifications added by specific enzymes to regulate gene expression.

p.1
Epigenetics and Gene Expression

What is the function of DNA methyltransferases (DNMTs)?

They synthesize new methylation marks for DNA methylation.

p.2
Structure of B-DNA

What is the most common conformation of double-stranded DNA?

B-DNA.

p.2
Structure of B-DNA

What are the major and minor grooves in B-DNA?

Major is the larger gap; minor is the smaller gap.

p.2
Structure of B-DNA

What type of helix is B-DNA?

Right-handed helix.

p.2
Eukaryotic Transcription Initiation

What happens during the elongation phase of transcription?

Free RNA nucleotides are added to the template strand in the 5' to 3' direction.

p.2
Eukaryotic Transcription Initiation

What forms the transcription initiator complex in eukaryotic transcription?

TFII components and RNA polymerase II bind to the promoter region.

p.2
Structure of B-DNA

What does Chargaff's Rule state about double-stranded DNA?

%T = %A and %C = %G.

p.2
Structure of B-DNA

What composes the backbone of B-DNA?

<p>Ribose and phosphate.</p>

p.2
Structure of B-DNA

Which bases are found on the inside of B-DNA?

Adenine, thymine, guanine, cytosine.

p.2
Eukaryotic Transcription Initiation

Why is it easier for the TATA-box binding protein to bind to T and A?

Because there are only 2 hydrogen bonds between them.

p.2
Splicing of Pre-RNA

What is the role of the spliceosome complex in splicing?

It removes introns using small nuclear ribonucleoproteins (snRNPs) and RNA.

p.2
Splicing of Pre-RNA

What modifications occur to mRNA after splicing?

It is capped at the 5’ end and receives a poly-A tail at the 3’ end.

p.2
Eukaryotic Transcription Initiation

What triggers termination in transcription?

When a 'stop' sequence is reached or occurs randomly after the transcribed gene.

p.2
Eukaryotic Transcription Initiation

What is the role of the TATA-box binding protein in eukaryotic transcription initiation?

It binds to the DNA at the TATA box sequence.

Study Smarter, Not Harder
Study Smarter, Not Harder