How many NADH are produced during pyruvate oxidation?
2 NADH (1 per pyruvate).
What is the sulfate ion formula?
SO4^2-.
1/74
p.6
Glycolysis and ATP Production

How many NADH are produced during pyruvate oxidation?

2 NADH (1 per pyruvate).

p.1
Classification of Microorganisms

What is the sulfate ion formula?

SO4^2-.

p.1
Classification of Microorganisms

What is the nitrite ion formula?

NO2-.

p.4
Types of Microorganisms Based on Energy Sources

What are phototrophs?

Microorganisms that use light as an energy source.

p.6
Glycolysis and ATP Production

What reaction occurs when G3P is oxidized in glycolysis?

G3P is oxidized to 1,3-BPG, reducing NAD⁺ to NADH.

p.6
Glycolysis and ATP Production

How many ATP are consumed in the hexokinase reaction?

1 ATP.

p.3
Types of Microorganisms Based on Energy Sources

Give an example of heterotrophic organisms.

Fungi and animals.

p.3
Classification of Microorganisms

What is an important functional group of Acetyl CoA?

Thiol group.

p.1
Photosynthesis and Cellular Respiration

What is the photosynthesis reaction?

6 CO2 + 6 H2O (+ light) → C6H12O6 + 6 O2.

p.4
Types of Microorganisms Based on Energy Sources

What are chemoorganotrophs?

Chemotrophs that use organic molecules as an energy source.

p.4
Types of Microorganisms Based on Energy Sources

What are autotrophs?

Microorganisms that use CO₂ as a carbon source (self-feeding).

p.6
Glycolysis and ATP Production

What is the product of the phosphofructokinase reaction?

Fructose-1,6-bisphosphate.

p.4
Types of Microorganisms Based on Energy Sources

What are lithotrophs?

Microorganisms that use inorganic compounds as an electron donor.

p.1
Photosynthesis and Cellular Respiration

What is the cellular respiration reaction?

C6H12O6 + 6 O2 → 6 CO2 + 6 H2O + energy.

p.1
Free Energy and Thermodynamics

What is the formula for free energy (Gibbs-Helmholtz equation)?

ΔG = ΔH - TΔS.

p.1
Classification of Microorganisms

What is the nitrate ion formula?

NO3-.

p.2
Phosphoanhydride Bonds and Energy Transfer

Give an example of a group transfer reaction involving ATP.

ATP transfers a phosphoryl group to glucose, producing glucose-6-phosphate and ADP.

p.5
Types of Microorganisms Based on Energy Sources

What is a chemoheterotroph?

An organism that derives both energy and carbon from organic compounds.

p.5
Phosphoanhydride Bonds and Energy Transfer

What is a phosphoanhydride bond?

A high-energy bond between phosphate groups in molecules like ATP, powering essential cellular processes.

p.3
Types of Microorganisms Based on Energy Sources

How do you categorize a microorganism that uses CO₂ and H₂O to make a living?

Autotroph.

p.4
Types of Microorganisms Based on Energy Sources

What are chemolithotrophs?

Chemotrophs that use inorganic molecules as an energy source.

p.1
Photosynthesis and Cellular Respiration

What drives life in the oceans?

Light triggers phytoplankton to start photosynthesis, initiating the food web.

p.4
Types of Microorganisms Based on Energy Sources

What are organotrophs?

Microorganisms that use organic compounds as an electron donor.

p.2
Free Energy and Thermodynamics

What is the equation that relates Gibbs free energy (ΔG), temperature (T), entropy (ΔS), and enthalpy (ΔH)?

ΔG = -T ∙ ΔS + ΔH.

p.1
Free Energy and Thermodynamics

What is the energetical classification of processes based on ΔG?

ΔG<0: exergon (spontaneously feasible), ΔG=0: reversible (equilibrium), ΔG>0: endergon (not spontaneously feasible).

p.6
Glycolysis and ATP Production

How many CO₂ molecules are released during the citric acid cycle?

4 CO₂.

p.2
Glycolysis and ATP Production

Why are there 36 ATP formed during cellular respiration?

1 mole NADH = 3 mole ATP, 1 mole FAD = 1.5 mole ATP, with contributions from glycolysis, decarboxylation of pyruvate, and the citric acid cycle.

p.2
Redox Reactions in Metabolism

What is a redox reaction?

A reaction where two redox pairs work together.

p.5
Catabolic Pathways in Microorganisms

What is aerobic respiration?

A process that uses oxygen as an electron acceptor, producing a large amount of ATP.

p.5
Energy Carriers in Microorganisms

What is electron transport phosphorylation (ETP)?

A process in aerobic respiration where a proton motive force is generated by electron transport, indirectly forming ATP.

p.3
Types of Microorganisms Based on Energy Sources

What are photoautotrophs?

<p>Microorganisms that use light energy to convert CO₂ and H₂O into organic compounds via photosynthesis. Example: Cyanobacteria.</p><p></p>

p.6
Glycolysis and ATP Production

Which enzyme catalyzes the conversion of glucose to glucose-6-phosphate?

Hexokinase.

p.3
Types of Microorganisms Based on Energy Sources

What is heterotrophic?

Organisms that cannot produce their own food and must obtain energy and carbon from organic compounds from other organisms.

p.1
Microbial Distribution in Oceans

What is the average depth of the ocean?

4000 meters.

p.6
Glycolysis and ATP Production

What are the main achievements of glycolysis?

Oxidation of substrate to pyruvate, production of 4 ATP, and 4 reducing equivalents.

p.1
Free Energy and Thermodynamics

What is the definition of Free Energy?

ΔG, the portion of energy available to do work, determines how systems change.

p.6
Glycolysis and ATP Production

What does the citric acid cycle produce in terms of ATP?

2 ATP.

p.6
Glycolysis and ATP Production

What are the reducing equivalents generated in the citric acid cycle?

6 NADH and 2 FADH₂.

p.2
Phosphoanhydride Bonds and Energy Transfer

What is a group transfer reaction?

It involves the transfer of a functional group from one molecule (donor) to another (acceptor).

p.5
Types of Microorganisms Based on Energy Sources

What is a photoheterotroph?

An organism that obtains energy from light and carbon from organic compounds.

p.5
Catabolic Pathways in Microorganisms

What is chemolithotrophy?

A process that uses inorganic compounds for energy.

p.4
Types of Microorganisms Based on Energy Sources

What are chemotrophs?

Microorganisms that use chemical compounds as an energy source.

p.3
Energy Carriers in Microorganisms

What is lithotrophy?

The process of obtaining energy by oxidizing inorganic compounds as electron donors.

p.4
Types of Microorganisms Based on Energy Sources

What are heterotrophs?

Microorganisms that use organic compounds as a carbon source (feed on others).

p.6
Glycolysis and ATP Production

Which enzyme produces ATP from 1,3-bisphosphoglycerate?

Phosphoglycerate kinase.

p.4
Types of Microorganisms Based on Energy Sources

What is a photoautotroph?

An organism that gets energy from light, carbon from CO₂, and electrons from inorganic compounds (e.g., plants, cyanobacteria).

p.6
Glycolysis and ATP Production

What is formed during pyruvate oxidation?

Acetyl-CoA.

p.2
Free Energy and Thermodynamics

What is the unit of absolute temperature (T)?

Kelvin.

p.2
Energy Carriers in Microorganisms

Name some energy-rich carriers.

ATP, ADP, Acetyl-CoA.

p.1
Classification of Microorganisms

What is the formula for glucose?

C6H12O6.

p.5
Types of Microorganisms Based on Energy Sources

What is a chemoautotroph?

An organism that derives energy from inorganic chemicals, carbon from CO₂, and electrons from inorganic sources.

p.5
Catabolic Pathways in Microorganisms

What is phototrophy?

A process that uses light to produce ATP.

p.5
Redox Reactions in Metabolism

Which reaction in glycolysis is a redox reaction?

The conversion of glyceraldehyde-3-phosphate (G3P) to 1,3-bisphosphoglycerate (1,3-BPG) catalyzed by glyceraldehyde-3-phosphate dehydrogenase.

p.3
Types of Microorganisms Based on Energy Sources

What are chemoautotrophs?

Microorganisms that use chemical energy from inorganic substances to convert CO₂ and H₂O. Example: Certain bacteria in extreme environments.

p.3
Types of Microorganisms Based on Energy Sources

Give an example of a microorganism that uses lithotrophy.

Methanogens (archaea) that use H₂ as donor and CO₂ as acceptor to make methane.

p.1
Microbial Distribution in Oceans

What effect does ocean current have on microorganisms?

It prevents isolation and distributes microorganisms.

p.6
Glycolysis and ATP Production

What is produced in the pyruvate kinase reaction?

ATP and pyruvate.

p.2
Free Energy and Thermodynamics

What does enthalpy (ΔH) represent?

The total heat content of a system, reflecting the number and kinds of bonds.

p.2
Free Energy and Thermodynamics

What does entropy (ΔS) measure?

The degree of randomness in a system.

p.2
Energy Carriers in Microorganisms

How can microorganisms make endergonic reactions possible?

By using energy from light during photosynthesis.

p.2
Energy Carriers in Microorganisms

Name some moderate energy carriers.

AMP, G-3-P.

p.2
Energy Carriers in Microorganisms

Which enzyme class drives phosphorylation?

Kinase, which catalyzes phosphoryl group transfers with ATP as the donor.

p.5
Catabolic Pathways in Microorganisms

What is fermentation?

A catabolic process that does not use an external electron acceptor and yields low ATP from organic molecules.

p.5
Free Energy and Thermodynamics

Why does a cell require -70 to -80 kJ mol-1 for ATP synthesis?

Because actual cellular conditions differ from standard laboratory conditions, leading to heat loss and lower efficiency.

p.2
Glycolysis and ATP Production

What are the most important reactions that occur during glycolysis?

ATP synthesis and oxidation-reduction reactions.

p.5
Catabolic Pathways in Microorganisms

What is anaerobic respiration?

A process that uses inorganic molecules instead of oxygen, producing less ATP.

p.5
Energy Carriers in Microorganisms

What is substrate level phosphorylation (SLP)?

A process that directly generates ATP by transferring a phosphate group from a high-energy substrate molecule to ADP.

p.5

Which two basic types of reaction exist for conserving energy?

<p>Substrate level phosphorylation and Electron transport phosphorylation</p>

p.6

Which reactions in glycolysis yield (produce) ATP?

<ul class="tight" data-tight="true"><li><p>Phosphoglycerate kinase reaction:</p><ul class="tight" data-tight="true"><li><p>1,3-bisphosphoglycerate + ADP → 3-phosphoglycerate + ATP</p></li></ul></li><li><p>Pyruvate Kinase reaction:</p><ul class="tight" data-tight="true"><li><p>phosphoenolpyruvate + ADP → pyruvate + ATP</p></li></ul></li></ul><p></p>

p.6

Which reactions in glycolysis consume (use) ATP?

<ul class="tight" data-tight="true"><li><p>Hexokinase Reaction: </p><ul class="tight" data-tight="true"><li><p>Glucose + ATP → Glucose-6-phosphate + ADP</p></li></ul></li><li><p>Phosphofructokinase reaction: </p><ul class="tight" data-tight="true"><li><p>Fructose-6-phosphate + ATP → Fructose-1,6-bisphosphate + ADP</p></li></ul></li></ul><p></p>

p.6

What are the achievements of glycolysis?

<ul class="tight" data-tight="true"><li><p>Oxidation of the substrate to pyruvate</p></li><li><p>4 reducing equivalents</p></li><li><p>4 ATP per SLP</p></li></ul><p></p>

p.6

What are the achievements of the citric acid cycle?

<ul class="tight" data-tight="true"><li><p>2 ATP</p></li><li><p>6 NADH</p></li><li><p>2 FADH2</p></li><li><p>4 CO2 released</p></li></ul><p></p>

p.6

What are the achievements of pyruvate oxidation?

<ul class="tight" data-tight="true"><li><p>Acetyl-CoA formation</p></li><li><p>2 NADH produces</p></li><li><p>2 CO2 released</p></li></ul><p></p>

p.5

What options do microorganisms have in catabolism? Name 5

<ol class="tight" data-tight="true"><li><p>Fermentation</p></li><li><p>Anaerobic respiration</p></li><li><p>Aerobic respiration</p></li><li><p>Photography</p></li><li><p>Chemolithotrophy</p></li></ol><p></p>

Study Smarter, Not Harder
Study Smarter, Not Harder